PHYLOGENETIC AFFINITY OF RAT AND SOME MAMMALIAN SPECIES METALLOTHIONEIN GENES
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Ключові слова

metallothionein, phylogenetic affinity, gene, isoform, coding sequence, alignment, phylogenetic tree.

Як цитувати

Peka, M., Balatsky, V., Korinnyi, S., & Saienko, A. (2021). PHYLOGENETIC AFFINITY OF RAT AND SOME MAMMALIAN SPECIES METALLOTHIONEIN GENES. ГРААЛЬ НАУКИ, (6), 103-108. https://doi.org/10.36074/grail-of-science.25.06.2021.019

Анотація

Metallothioneins ( are low molecular weight proteins, which have ability to bind both
mono and divalent metals and to form metal protein complexes. In mammals, MT are presented
by four isoforms (MT 1, MT 2, MT 3 and MT 4), which are encoded by the family of metallotionein
genes. The purpose of the present study was to determine the p hylogenetic affinity of different rat
Rattus norvegicus MT genes with other mammalian spe cies, as well as to determine the degree of
similarity between genes of MT isoforms within s pecies Rattus norvegicus. Therefore nucleotide
sequences of MT genes were aligned, the percentage of identity and the number of gaps was
counted and a phylogenetic analysis was carrie d out. For genes of all Rattus norvegicus MT
isoforms, significant similarity with other ma mmalian species Mus musculus Homo s ap iens Sus
scrofa was shown. The coding sequences ( of genes of Rattus norvegicus MT 1, MT 2A, MT 3
and MT 4 isoforms have a significant similarity among themselves. The MT genes are hig hly
conservative, both at the interspecific and intraspecific levels, which is probably associated with
their biological functions.

https://doi.org/10.36074/grail-of-science.25.06.2021.019
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Посилання

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